It's widely acknowledged that variants within ncRNAs have the potential to alter their secondary structures, consequently impacting the biogenesis process. To explore this phenomenon, we assessed the influence of wild type and MNV mutant on the secondary structure of pre-miRNAs using RNAfold. Our findings revealed that 396 MNVs presented in pre-miRNAs, and 287 ones (72.47%) could cause differences in the secondary structure of pre-miRNAs (|Δ Minimum Free Energy (ΔMFE)| > 1). Additionally, we employed RNAsnp to analyze discrepancies in the secondary structure of lncRNAs before and after mutations. Our results indicated that a total of 145,234 MNVs could potentially impact the secondary structure of lncRNAs (p value < 0.2).
MNVncRNAStrucdb consists of four modules: . On the 'Search' page, users can query the impacts of MNV on ncRNA structures by entering the corresponding information. On the 'Predict' page, users can conduct online predictions regarding the effects of MNV on ncRNA structures between wild-type and mutated sequences. On the 'Graph' page, users can visualize the structure of ncRNAs by inputting sequence and structure information obtained from RNAfold. On the 'Download' page, users can download related information.
In MNVncRNAStrucdb, you can:
Query the effects of MNVs on ncRNA structures predicted by different methods.
Visualize the effects of MNVs through a sequence graph.
Perform online predictions of the effects of MNVs submitted by users.
Download differential results.